Detection & Diagnostics

ATP Bioluminescence

applications of Bacteria detection



Detection of Bacterial in Food




Detection of Bacteria in Biodenfense



ATP Bioluminescence

BTC is working collaboratively in the area of Bioluminescence - Detection of ATP from bacterial cells. The first assay is related to a generic bacterial detection system to determine general contamination. Through these efforts, we can identify specific bacteria with the inclusion of specific phage lytic enzymes (gram positive lytic enzyme detection).

The bioluminometer shown here is able to determine the presence of low levels of viable bacteria and as few as 100 spores. The system generally provides results within five minutes for bacterial detection and within 20 minutes for spore detection.


  • FDA 510k Issued For Human Samples

  • DOD Validated

  • Validated For Carcass Contamination By USDA

  • Validated For Water Analysis By WHO Reference Laboratory

  • > 90% Correlation To Culture

  • Differentiate Bacterial And Non-bacterial Sources of ATP

  • Remove Interfering Substances & Free ATP

Plague Diagnostics Development

BTC is in the process of developing a rapid point-of-care assay to diagnose plague infections. This work is supported by US DOD to help reduce front line casualties from natural infections or possible bioweapons. This assay will be a multiplex lateral flow assay that detects two antigens from the bacteria causative for plague. The picture shows preliminary test results with purified antigens. With this assay, a test can be performed in 15 to 20 minutes with a drop of blood from a finger stick.


hand held assay (hha)

The SMART assay was the first rapid hand held assay developed in 1986 by the NHD team.  In a new advancement to Hand Held Assays, the polymer enhanced SMART lateral flow assay provides an increase of up to 1 log in sensitivity as part of the first bio-defense test used since Desert Storm I in 1991. This new assay can be used for the detection of unknown bacteria, viruses and toxins for defense, food, animal or environmental applications.

Desert Storm
During the Gulf War we deployed 17 research and development systems to the Gulf region to monitor the air for suspected Iraqi Biological Warfare (BW) agents. Twelve of the 17 systems were mobile, and consisted of the Army’s XM-2 high volume air samplers and Sensitive Membrane Antigen Rapid Test (SMART) identification tickets.
— General Norman Schwarzkopf

selected Lateral Flow Assays (smart ii)

Enhanced lateral flow technology with polymer-enhanced gold nanoparticles and optimized other parameters including protein concentrations, solution components and treatment conditions.

Polymer of Enhancement of Gold Nanoparticle Conjugation with Antibody

Polymer Enhancement of Gold Nanoparticle Conjugation With Antibody






Anthrax Spore

Anthrax Spore

Rapid Detection

BTC and NHDetect are teaming to offer products focused on the rapid detection of bacteria, viruses and toxins. 

Medical Applications

Biothreat Detection

Diagnostic Kits

Collection Kits


Food Testing

Fluorescence Immunoassay System

Fluorescence Immunoassay System

BTC also is working with partners on the development of a Membrane Enhanced Fluorescence Immunoassay System with specific monoclonal antibodies for the rapid and sensitive detection of bacteria, viruses and toxins based on immunological (antibody-antigen) reactions. A combined 180 degree optical/membrane configuration will be employed for rapid, high sensitivity and simple determination of low concentrations of target substances such as bacteria, spores and viruses.

The fluorescence antibody mixture is immobilized on a carefully selected translucent membrane with appropriately low fluorescence background and high sensitivity characteristics.

Operational Capability

  • < 1 lb, 6" x 3" x 1.5", Battery Powered

  • Glucose Like Strip 2 Step Method < 3 minutes Total Time

  • Stability > 1 Year

  • Sensitivity Equal To or Greater Than Current Methods

  • Rugged Field Usable

  • Download And Data Configuration Capable (Future)